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Antibody(GTP binding proteins)|一般社団法人 日本細胞生物学会

一般社団法人
日本細胞生物学会Japan Society for Cell Biology

Antibody(GTP binding proteins)

【A】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
ARF2011-08-29 H-50WBIF IHCIP
Santa Cruzx200-1000
rabbit
Poly

Hela細胞
ARF52011-08-29 M01WBIF IHCIP
Abnova
mouse
Mono, IgG1

Hela細胞
Arfaptin22011-08-29 2B5WBIF IHCIP
Abnovax1000x200-500
mouse
【PFA+Triton】:◎【PFA条件】3%PFA/PBS 37℃10min【Triton条件】0.1% Triton-X100/PBS
Mono, IgG2b

Hela細胞
ARF2011-08-29 1D9WBIF IHCIP
ABRx500x500
mouse
【PFA+Triton】:×,【IS】:○?【PFA条件】3%PFA/PBS 37℃10min【Triton条件】0.1% Triton-X100/PBS
Mono, IgG1

Hela細胞
ARF12011-08-29 3F1WBIF IHCIP
ABRx200
mouse
mono, IgG2a

Hela細胞
ARF32011-08-29 WBIF IHCIP
BD Transductionx200
mouse
【PFA+Triton】:×,【IS】:○?【PFA条件】3%PFA/PBS 37℃10min【Triton条件】0.1% Triton-X100/PBS
Mono, IgG1

Hela細胞
ARF42011-08-29 WBIF IHCIP
PTG
rabbit
Poly

Hela細胞
ARF62011-08-29 3A-1WBIF IHCIP
Santa Cruzx2000x200
mouse
【PFA+Triton】:×,【IS】:○?,【TCA】:○【PFA条件】3%PFA/PBS 37℃10min【TCA条件】TCA 10%/H2O on ice 15min【Triton条件】0.1% Triton-X100/PBS
Mono, IgG2b
IFはTCA固定
TCA fix is best for IF.
Hela細胞
Arfaptin12011-08-29 I-19WBIF IHCIP
Santa Cruzx1000x100
goat
【PFA+Triton】:○,【PFA+Saponin】:○【PFA条件】3%PFA/PBS 37℃10min【Triton条件】0.1% Triton-X100/PBS【saponin条件】0.05% saponin /PBS
Poly
WB:バンドが二本
Two bands appear by WB.
Hela細胞
Arfaptin12011-08-29 N-20WBIF IHCIP
Santa Cruzx1000x100
goat
【PFA+Triton】:○,【PFA+Saponin】:○【PFA条件】3%PFA/PBS 37℃10min【Triton条件】0.1% Triton-X100/PBS【saponin条件】0.05% saponin /PBS
Poly
WB:バンドが一本、お勧め
A unique band appear by WB. Recommended Ab.
Hela細胞
Arfaptin22011-08-29 WBIF IHCIP
Zymedx1000x100
rabbit
【PFA+Triton】:○,【PFA+Saponin】:○【PFA条件】3%PFA/PBS 37℃10min【Triton条件】0.1% Triton-X100/PBS【saponin条件】0.05% saponin /PBS
Poly

Hela細胞
ATGL2011-08-29 WBIF IHCIP
Caymanx100
rabbit
【PFA+Triton】:×,【PFA+Saponin】:×,【MeOH 】:×,【PFA+70%EtOH 】:×,【PFA_digitonin】:△(Can Get B)【PFA条件】3%PFA/PBS 37℃10min【MeOH条件】MeOH 100% -20℃ 5min【Triton条件】0.1% Triton-X100/PBS【saponin条件】0.05% saponin /PBS【digitonin条件】50ug/ml digitonin /PBS
Poly

Hela細胞
AF-62011-08-14 WBIF IHCIP
BD transduction
610732
mouse
m

ARF12011-10-04
About Product
MonoclonalWBIF IHCIP
AbcamX1000
ab11038
mouse
IgG2a

HL-60細胞
ASAP12011-08-29
About Product
PolyclonalWBIF IHCIP
Abcam
ab64185
rabbit
IgG

Hela細胞
human ARF62011-08-10 3A-1WBIF IHCIP
Santa Cruzx100
sc7971
3%PFA (in PBS), methanol
mouse
m

マウス小腸

【B】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
BIG12011-10-04 WBIF IHCIP
Santa CruzX500
sc-50391
rabbit
Poly

HL-60細胞

【C】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
Cdc422011-10-17 44/CDC42WBIF IHCIP
BD TransductionX1000
610928
mouse
Mono,IgG1
WB:Blocking/3%BSA-TBS, 1stAb/3%BSA-TBS, 2ndAb/3% skin milk-TBSが良い。
HeLa細胞
cdc422011-10-28 WBIF IHCIP
BD transduction 1/250
610928
mouse
m
WBはcan get signal (TOYOBO)使用
Use Cangetsignal (TOYOBO) for WB
Cdc422011-09-27 WBIF IHCIP
BD100
610929
mouse
m
TCAのみOK
Visualized only by TCA fix.
MDCK II,HeLa, DLD-1, EpH4, DLD-1, ニワトリ胚マウス胚のプラコード
Cdc422011-09-27 WBIF IHCIP
Cytoskeleton100
ACD01
rabbit
p
弱すぎる
Too weak staining.
MDCK II
Cdc422011-09-27 WBIF IHCIP
Santa Cruz100
sc-6083
goat
p
蛍光染色不可
Not suitable for IF.
MDCK II
Cdc422011-09-27 WBIF IHCIP
Santa Cruz100
sc-8401
mouse
m
蛍光染色不可
Not suitable for IF.
MDCK II
Cdc422011-10-04 B-8WBIF IHCIP
Santa CruzX500
sc-8401
mouse
Mono,IgG3

HL-60細胞
Cdc422011-09-27 WBIF IHCIP
Santa Cruz100
sc-87
rabbit
p
蛍光染色不可
Not suitable for IF.
MDCK II

【F】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
FIP32011-08-29 WBIF IHCIP
Rockland
rabbit
Poly

Hela細胞

【G】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
GBF12011-08-29 WBIF IHCIP
BD Transductionx100
mouse
【PFA+Triton】:◎,【MeOH】:△,【PFA_70%EtOH】:○【PFA条件】3%PFA/PBS 37℃10min【MeOH条件】MeOH 100% -20℃ 5min【Triton条件】0.1% Triton-X100/PBS
Mono, IgG1
MDCKII OK.
Hela細胞
Gb2011-10-04 WBIF IHCIP
UpstateX500
06-238
rabbit
Poly

HL-60細胞
GBF12011-10-04 25WBIF IHCIP
BD TransductionX1000X100
612116
mouse
【4%PFA+0.2%Saponin】:○
Mono, IgG1

HL-60細胞

【R】

Antigen
last update
Clone
Application outlook for label
MakerDilution rate
Catalog codeFixation condition for IF and IHC
HostFixation and permeabilization condition
Isotype
Comments
Samples
Reference
Rab72011-08-14 WBIF IHCIP
MBL
rabbit
p

Rabaptin52011-08-29 C-20WBIF IHCIP
Santa Cruzx500-1000x50
goat
Poly

Hela細胞
Rabaptin52011-08-29 N-19WBIF IHCIP
Santa Cruz
goat
Poly

Hela細胞
Rabaptin-52011-08-29 clone 20WBIF IHCIP
BD Transductionダメ
mouse
【PFA+Triton】:×,【PFA+Saponin】:×,【IS】:×,【MeOH】:×【PFA条件】3%PFA/PBS 37℃10min【MeOH条件】MeOH 100% -20℃ 5min【Triton条件】0.1% Triton-X100/PBS【saponin条件】0.05% saponin /PBS
Mono, IgG1

Hela細胞
Rab112011-08-29 WBIF IHCIP
Zymedx500x100-
rabbit
【PFA+Triton】:△,【PFA+Saponin】:○,【TCA】:○【PFA条件】3%PFA/PBS 37℃10min【TCA条件】TCA 10%/H2O on ice 15min【Triton条件】0.1% Triton-X100/PBS【saponin条件】0.05% saponin /PBS
Poly
WB:Rab11aのみ認識、IFで見えるendosomeのシグナルはKDで半分ぐらいしか消えない。
Only recognize Rab11a by WB. Only half of the endosome staining dissapears in Rab11a KD cells.
Hela細胞
Rab112011-08-29 clone 47WBIF IHCIP
BD Transductionx200-x200
mouse
【PFA+Triton】:×,【PFA+Saponin】:×,【IS】:×,【MeOH】:×,【TCA】:○、◎(+CanGetSignal (from TOYOBO Inc.))、BlockingはO/N【PFA条件】3%PFA/PBS 37℃10min【MeOH条件】MeOH 100% -20℃ 5min【TCA条件】TCA 10%/H2O on ice 15min【Triton条件】0.1% Triton-X100/PBS【saponin条件】0.05% saponin /PBS
Mono, IgG2a
WB: Rab11a, b両方認識する
Recognize both Rab11a and Rab11b by WB.
Hela細胞
Rab 5B2011-08-29 A-20WBIF IHCIP
Santa Cruz
rabbit
Poly

Hela細胞
Rab 5A2011-08-29 sc-309WBIF IHCIP
Santa Cruzx100
rabbit
Poly

Hela細胞
Rab52011-08-29 clone 1WBIF IHCIP
BD Transductionx200-x100-
mouse
【PFA+Triton】:○,【PFA+Saponin】:○,【IS】:×,【MeOH】:○【PFA条件】3%PFA/PBS 37℃10min【MeOH条件】MeOH 100% -20℃ 5min【Triton条件】0.1% Triton-X100/PBS【saponin条件】0.05% saponin /PBS
Mono, IgG2a
PFAでもいいが、saponinがbetter
Saponin permeabilization is better.
Hela細胞
Rab 42011-08-29 D-20WBIF IHCIP
Santa Cruz
rabbit
Poly

Hela細胞
Rab42011-08-29 clone 7WBIF IHCIP
BD Transductionx100-ダメ
mouse
【PFA+Triton】:×,【PFA+Saponin】:×,【IS】:×,【MeOH】:×【PFA条件】3%PFA/PBS 37℃10min【MeOH条件】MeOH 100% -20℃ 5min【Triton条件】0.1% Triton-X100/PBS【saponin条件】0.05% saponin /PBS
Mono, IgG1
endosome likeのsignalが見えるが、siRab4で消えない。
Recognize endosome-like signals which cannot disappear by siRab4.
Hela細胞
Rac12011-08-29 WBIF IHCIP
Chemicon
mouse
Mono

Hela細胞
Rac12011-08-29 clone 102WBIF IHCIP
BD Pharmingenダメ
mouse
【PFA+Triton】:×,【PFA+Saponin】:×,【IS】:×,【MeOH】:×【PFA条件】3%PFA/PBS 37℃10min【MeOH条件】MeOH 100% -20℃ 5min【Triton条件】0.1% Triton-X100/PBS【saponin条件】0.05% saponin /PBS
Mono, IgG2b

Hela細胞
RhoA/C2011-09-27 Lulu51WBIF IHCIP
Yonemura50-100
rat
m

HeLa, MDCK II, EpH4, 3Y1, P3, L, ほとんどのマウス組織
RhoA2011-09-27 おそらく26C4と同クローン (probably clone 26C4)WBIF IHCIP
ARP100
01-4301M
mouse
m
TCA おそらく26C4ど同クローン
HeLa
Rac12011-09-27 clone 23A8WBIF IHCIP
Upstate100
05-389
mouse
m
4%PFA, TCA
HeLa, MDCK II, DLD-1, EpH4, MTD-1A
Rac12011-10-04 23A8WBIF IHCIP
MilliporeX2000
05-389
mouse
Mono, IgG2b

HL-60細胞
RhoABC2011-09-27 clone 55WBIF IHCIP
Upstate100
05-778
mouse
m
TCA
HeLa
Rac22011-10-04 WBIF IHCIP
UpstateX5000
07-604
rabbit
Poly

HL-60細胞
Rab352011-08-31 WBIF IHCIP
ProteinTech Group
11329-2-AP
rabbit
p

Rho (N-term)2011-09-27 WBIF IHCIP
EPT100
1600-1
rabbit
m
TCA 染色は弱い
Weak staining
HeLa
Rho2011-09-27 clone EP487YWBIF IHCIP
EPT100
1667-1
rabbit
m
分裂溝はTCAのみだが弱い
Cleavage furrows can be weakly stained only by TCA fix.
HeLa
ROCK12011-09-27 clone EP786YWBIF IHCIP
EPT100
1761-1
rabbit
m
分裂溝の染色はTCAのみ
Cleavage furrows can be stained only by TCA fix.
HeLa
Rab272011-08-31 WBIF IHCIP
IBL
18975
4%PFA (in PBS)
rabbit
p
Rab27A,B共に認識
recognize both Rab27a and Rab27b
melan-a
Rab72011-08-31 WBIF IHCIP
Cell signaling
2094S
4%PFA (in PBS)
rabbit
p

melan-a
RhoA (67B9)2011-09-27 WBIF IHCIP
Cell Signaling100
2117
rabbit
m
TCA
HeLa
Rab82012-04-17 4/Rab4WBIF IHCIP
Transductionx5,000~
61-844
mouse
IgG2b

Ran2011-10-13 20WBIF IHCIP
BDX2000~
610340
mouse
PFA+Triton or MeOH or TCA
Mono, IgG2a

HeLa細胞
Rab32011-08-31 WBIF IHCIP
BD transduction
610379
4%PFA (in PBS)
mouse
m

マウス海馬ニューロン, PC12
Rab272011-08-14 WBIF IHCIP
BD transduction
610596
mouse
m

Rab112011-08-14 WBIF IHCIP
BD transduction
610656
mouse
m

Rab112011-08-31 WBIF IHCIP
BD transduction
610656
4%PFA (in PBS)
mouse
m
Rab11A,B共に認識
recognize both Rab11a and Rab11b
MEF
Rab112011-09-27 clone 47/Rab11WBIF IHCIP
BD100
610656
mouse
m
TCA (Can Get Signal Immunostain 使用)
http://www.toyobo.co.jp/seihin/xr/lifescience/tech/upload/upld96/technical/canget96tr01.pdf
http://www.toyobo.co.jp/seihin/xr/lifescience/tech/upload/upld95/technical/canget95tr02.pdf
HeLa
Rab82011-08-14 WBIF IHCIP
BD transductionx1000x100
610845
3%PFA (in PBS), methanol
mouse
m
Rab8a,b共に認識
Recognize both Rab8a and Rab8b
マウス小腸
Rab112011-08-14 WBIF IHCIP
Zymed
71-5300
rabbit
p

Rab112011-08-31 WBIF IHCIP
Invitrogen
71-5300
4%PFA (in PBS)
rabbit
p
Rab11A,B共に認識
Recognize both Rab11a and Rab11b
マウス海馬ニューロン, MEF
Rabenosyn-52011-08-29
About Product
PolyclonalWBIF IHCIP
Abcamダメ?ダメ
ab21196
goat
【PFA+Triton】:×,【PFA+Saponin】:×,【IS】:×,【MeOH】:×【PFA条件】3%PFA/PBS 37℃10min【MeOH条件】MeOH 100% -20℃ 5min【Triton条件】0.1% Triton-X100/PBS【saponin条件】0.05% saponin /PBS
IgG

Hela細胞
Rab142011-08-29
About Product
PolyclonalWBIF IHCIP
Abcamx500x100
ab40938
rabbit
IgG
Saponinがbetter 汚い
Saponin permeabilization is better.
Hela細胞
RhoA2011-09-27 おそらく26C4と同クローン (probably clone 26C4)WBIF IHCIP
Cytoskeleton100
ARH01
m
m
TCAのみOK
Visualized only by TCA fix.
HeLa,MDCK II
Rab33B2011-08-31 WBIF IHCIP
Frontier Science
D5
4%PFA (in PBS)
mouse
m
MEF, NIH3T3ではIF不可。Rab33Aは認識せず。
Not suitable for IF in MEFs and 3T3. Not recognize Rab33A.
HeLa, HeLaS3
Rab1A2011-08-31 WBIF IHCIP
Abnova
H00005861-M07A
mouse
m

Rab27A2011-08-31 WBIF IHCIP
Abnova
H00005873-M02
mouse
m

rab33a2011-10-03 8A11WBIF IHCIP
Abnovax1000
H00009396-M04
mouse
【Formalin in PBS+0.05%Triton】:○,
【Formalin in Ringer+0.05%Triton】:○
Mono,IgG2ak

ホルマリンは37%ホルマリン液(ナカライ)をPBSやRingerで1/10に希釈
Formalin solution is made by dilution of 37% formalin (Nacalai Inc.) by PBS or Ringer's solution
rat hippocampal neurons
RACGAP12011-09-27 clone 1G6WBIF IHCIP
Abnova100
H00029127-M01
m
m
MeOHでmidobodyのみわかる程度で弱い
Only midbody can be weakly visualized by MeOH fix.
HeLa
RACGAP1/MgcRacGAP2011-09-27 WBIF IHCIP
IMGENEX100
IMG-3464
goat
p
弱い(midbodyのみ)
Only midbody can be weakly visualized.
HeLa
Rab42011-08-14 WBIF IHCIP
Stressgen
KAP-GP005
rabbit
p

pan-Ras2013-09-12 Ab-3WBIF IHCIP
Oncogenex100
OP#40
mouse
IgG2a

HeLa
N-Ras2011-10-13 F115-227WBIF IHCIP
CALBIOCHEMX 500
OP25
mouse
Mono, IgG1

HeLa細胞
Rab52011-08-14 WBIF IHCIP
BD transduction
R28720
mouse
m

Rab72011-08-29 WBIF IHCIP
Sigmaダメ
R4779
rabbit
【PFA+Triton】:×,【PFA+Saponin】:×,【IS】:×,【MeOH】:×【PFA条件】3%PFA/PBS 37℃10min【MeOH条件】MeOH 100% -20℃ 5min【Triton条件】0.1% Triton-X100/PBS【saponin条件】0.05% saponin /PBS
Poly

Hela細胞
Rab82011-08-29 WBIF IHCIP
Sigmax300-500
R5530
rabbit
【PFA+Triton】:○,【TCA】:◎【PFA条件】3%PFA/PBS 37℃10min【TCA条件】TCA 10%/H2O on ice 15min【Triton条件】0.1% Triton-X100/PBS
Poly, anti-sera,IgGfraction

Hela細胞
Rho2011-09-27 WBIF IHCIP
BD100
R73920
m
m

HeLa
Rab72011-08-29 Rab7-117WBIF IHCIP
Sigmaダメ
R8779
mouse
【PFA+Triton】:×,【PFA+Saponin】:×,【IS】:×,【MeOH】:×【PFA条件】3%PFA/PBS 37℃10min【MeOH条件】MeOH 100% -20℃ 5min【Triton条件】0.1% Triton-X100/PBS【saponin条件】0.05% saponin /PBS
Mono, IgG2b

Hela細胞
Rcc12011-10-13 N-19WBIF IHCIP
Santa Cruz
sc-1161
goat
Poly

HeLa細胞
RhoA/B/C2011-09-27 WBIF IHCIP
Santa Cruz100
sc-179
rabbit
p
TCAのみOK
Visualized only by TCA fix.
HeLa, MECK II, ほとんどのマウス、ラット組織
ROCK-22011-09-27 WBIF IHCIP
Santa Cruz100
sc-1851
goat
p
4%PFA, 1%PFA, MeOH, TCA
DLD-1, EpH4
Rab82012-04-17 WBIF IHCIP
SantaCrusx500-1,000
sc-26578
goat

Rab5 (S-19)2011-10-17 WBIF IHCIP
Santa CruzX500
sc-309
rabbit
Poly
WB:Blocking/3%BSA-TBS, 1stAb/3%BSA-TBS, 2ndAb/3% skin milk-TBSが良い。
HeLa細胞
Rock-12011-09-27 WBIF IHCIP
Santa Cruz100
sc-6056
goat
p
1%PFA, TCA, MeOH
DLD?1
Rab6A2012-04-17 38-TBWBIF IHCIP
SantaCruzx1,000
sc-81913
mouse

human Rab72011-08-10 H-50WBIF IHCIP
Santa Cruzx100
sc10767
3%PFA (in PBS), methanol
rabbit
p

マウス小腸
human Rab62011-08-12 C-19WBIF IHCIP
Santa Cruz
sc310
rabbit
p
PC12細胞の免疫蛍光染色(3%PFA固定、0.05%saponinで膜のpermeabilize)よく染まった
Good staining for IF of PC12 with 3%PFA, 0.05%saponin
human Rab62011-08-10 C-19WBIF IHCIP
Santa Cruzx100
sc310
3%PFA (in PBS)
rabbit
p

マウスMEF、WB
human Rab6 (C-19)2012-08-03 WBIF IHCIP
Santa Cruzx100
sc310
3%PFA in PBS
rabbit
0.05% saponin

PC12
RhoA2011-09-27 26C4WBIF IHCIP
Santa Cruz100
SC418
m
m
TCAのみOK
Visualized only by TCA fix.
HeLa, MDCK II, EpH4, 3Y1, P3, L, ほとんどのラット組織
human Rab9A2011-08-10 C-18WBIF IHCIP
Santa Cruzx100
sc6562
3%PFA (in PBS), methanol
goat
p

マウス小腸
human Rab112011-08-10 C-19WBIF IHCIP
Santa Cruzx100
sc6565
3%PFA (in PBS), methanol
goat
p

マウス小腸